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Introdução: A irrupção de doenças emergentes como a COVID-19 causa uma série de alterações nas pessoas, incluindo enfermeiras, que estão na primeira linha de atendimento. Alguns dos efeitos incluem a presença de ansiedade, depressão, estresse, insônia e angústia. Objetivo: Avaliar o nível de incerteza sobre a doença COVID-19 no pessoal de enfermagem de segundo nível em Morelia, Michoacán, México. Metodologia: Estudo descritivo e transversal, em uma amostra de 160 enfermeiros de hospitais públicos ou privados de segundo nível de assistência, amostra de conveniência não-probabilística contatados via Facebook. Foram incluídos enfermeiros no atendimento direto a pacientes, utilizando a versão modificada da Escala de Incerteza de Doença e um questionário de dados sociodemográficos e ocupacionais. Foram usadas estatísticas descritivas e o qui-quadrado. O anonimato dos participantes foi respeitado. Resultados. A maioria dos participantes eram mulheres (87,5%), enfermeiras licenciadas (63,1%), idade média de 36,7 anos (SD=8,5) e 36,9% tinham estado em contato com pacientes da COVID-19. A incerteza total foi moderada em 56,9% e no pessoal de enfermagem em contato com os pacientes da COVID-19 foi de 64,8%. Foi encontrada uma relação estatisticamente significativa (p=≤0,05) entre incerteza e sexo, escolaridade; treinamento COVID-19 e uso de equipamento de proteção pessoal; disponibilidade de máscara facial e equipamento de proteção pessoal; fazer o curso COVID-19 e ter contato com pacientes COVID-19. Conclusões. Houve incerteza moderada na equipe de enfermagem dos hospitais de segundo nível, com diferenças nas variáveis pessoais, treinamento e disponibilidade de material e equipamento.
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